Fig. 1: RNA-seq analysis of SubAB-treated HeLa cells. | Cell Death Discovery

Fig. 1: RNA-seq analysis of SubAB-treated HeLa cells.

From: A novel endoplasmic stress mediator, Kelch domain containing 7B (KLHDC7B), increased Harakiri (HRK) in the SubAB-induced apoptosis signaling pathway

Fig. 1

HeLa cells were incubated with 400 ng mL−1 of SubAB wild-type (wt) or inactivated mutant (mt) for 18 h, followed by RNA extraction. RNA-seq analysis was performed using GENEWIZ. A Volcano plot of differentially expressed genes (DEGs). DEGs were identified using the DESeq2 Bioconductor package, which is based on the negative binomial distribution. Dispersion and logarithmic fold change estimates included data-driven prior distributions; Padj of genes were set at <0.05 to identify DEGs. B Gene ontology (GO) term enrichment analysis of DEGs; increased GO terms in biological process, molecular function, and cellular component branches are displayed.

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