Fig. 2: Knockdown of YBX2 increased BTB permeability in vitro.
From: Pseudogene RPL32P3 regulates the blood–tumor barrier permeability via the YBX2/HNF4G axis
A, B The mRNA and protein expression of YBX2 in AECs and GECs were detected by qRT-PCR and western blot. Data represent mean ± SD (n = 3, each). **P < 0.01 vs. AECs group. C, D Effects of YBX2 on TEER value and HRP flux. Data represent mean ± SD (n = 3, each). **P < 0.01 vs. YBX2(+)NC group, ##P < 0.01 vs. YBX2 (-)NC group. E Effects of YBX2 on the expression levels of ZO-1, occludin and claudin-5 determined by western blot. Data represent mean ± SD (n = 3, each). **P < 0.01 vs. YBX2(+)NC group, ##P < 0.01 vs. YBX2 (-)NC group. F Effects of YBX2 on ZO-1, occludin and claudin-5 expression levels and distribution determined by immunofluorescence. Scale bar represents 30 μm.
