Fig. 7: HNF4G bound to ZO-1, occludin and claudin-5 promoters, and inhibited their expression at the transcriptional level.
From: Pseudogene RPL32P3 regulates the blood–tumor barrier permeability via the YBX2/HNF4G axis
A–C Schematic diagrams of different reporter plasmids and relative dual-luciferase activities of ZO-1, occludin and claudin-5. The left side showed the deletion sites on the promoters of ZO-1, occludin and claudin-5, and the right side showed the decreases of reporter vector activities after the deletions of the putative binding sites of HNF4G. Data represent means ± SD (n = 5, each). **P < 0.01. D–F Schematic representations of ZO-1, occludin and claudin-5 promoter region from TSS to upstream 3000 bp in ChIP assays. TSS was designated as +1. Immunoprecipitated DNA was amplified by PCR. Normal rabbit IgG was used as a negative control.
