Fig. 1: Activation of apoptotic pathway in the optic nerve and retina of C57BL/6J mice after 24 h from eye removal. | Cell Death Discovery

Fig. 1: Activation of apoptotic pathway in the optic nerve and retina of C57BL/6J mice after 24 h from eye removal.

From: Retinal ganglion cell loss in an ex vivo mouse model of optic nerve cut is prevented by curcumin treatment

Fig. 1

A Graphical representation of experimental scheme performed to study the time-dependent retina neurodegeneration. After the eyeballs enucleation, optic nerves and retinal dissections were collected immediately after the sacrifice (time 0), after 6 (time 6), 12 (time 12), and 24 (time 24) hours from the sacrifice. B Representative western blots and relative quantifications of optic nerve lysates collected immediately after eye removal (time 0), after 6 h (time 6), 12 h (time 12), and 24 h (time 24). Graphs showed the significant time-dependent increase of cleaved-Caspase-3 level and decreased NeuN level in optic nerve lysates collected at time 24 in comparison to time 0. C Representative western blot and relative quantification of retinas lysates showed a significant augmented activation of Caspase-9, cleaved-Caspase-3, and BCL2 level at time 24 in comparison to time 0. The BCL-XL level was decreased in retinas collected at time 24 in comparison to time 0. Data were expressed as mean ± SEM. One-way ANOVA, Tukey’s post hoc test. *P < 0.05, **P < 0.01, and ***P < 0.001 [n = 5].

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