Fig. 2: Reduction of retinal ganglion cells and retinal cells vitality after 24 h from C57BL/6J mice eye removal. | Cell Death Discovery

Fig. 2: Reduction of retinal ganglion cells and retinal cells vitality after 24 h from C57BL/6J mice eye removal.

From: Retinal ganglion cell loss in an ex vivo mouse model of optic nerve cut is prevented by curcumin treatment

Fig. 2

A Representative western blot and relative quantification of retinas lysates showed a significant reduction of BRN3A [a specific marker for RGC nuclei] and NeuN level in retina lysates collected at time 24 in comparison to time 0. Data were expressed as mean ± SEM. One-way ANOVA, Tukey’s post hoc test. *P < 0.05, **P < 0.01, and ***P < 0.001 [n = 5]. B–G Immunofluorescence of retinal sections performed with BRN3A showed a reduction of BRN3A-positive cells (labeled in green) in retinas collected at time 24 (E–G) vs time 0 (B–D). Nuclei were stained with the nuclear marker DAPI (blue). GCL ganglion cell layer, IPL inner plexiform layer, INL inner nuclear layer, OPL outer plexiform layer, ONL outer nuclear layer. Scale bar: 20 μm. D, G Merged images at time 0 (D) and time 24 (G). D, G High magnification of merged images. Scale bar: 40 μm.

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