Fig. 5: Alterations of retinal layers thickness and influence of curcumin administration in induced retinal degeneration ex vivo model. | Cell Death Discovery

Fig. 5: Alterations of retinal layers thickness and influence of curcumin administration in induced retinal degeneration ex vivo model.

From: Retinal ganglion cell loss in an ex vivo mouse model of optic nerve cut is prevented by curcumin treatment

Fig. 5

A–E Representative image of hematoxylin & eosin (H&E) eye’s sections collected at time 0 (A) and magnification of retinal sections examined at time 0 (B), time 24 (C), 24 h pre-treated with 5 μM (D), and 10 μM (E) of curcumin depicting retinal morphological changes in H&E staining. GCL ganglion cell layer, IPL inner plexiform layer, INL inner nuclear layer, OPL outer plexiform layer, ONL outer nuclear layer, OS outer segment. Scale bar: 20 μm. F–K Measurement of retinal layer thickness in H&E eye sections collected at time 0, after 24 h from eye removal, 24 h pre-treated with 5 and 10 μM of curcumin. Graphs indicated at time 24 a reduction of ganglion cell layer (GCL, F), inner plexiform layer (IPL, G), inner nuclear layer (INL, H), outer nuclear layer (ONL, J), if compared to time 0. A tendency of increasing thickness was observed in samples pre-treated with curcumin in each layer analyzed, but resulted significant only for INL and ONL analysis (H, J); graph related to rod and cones inside ONL (R&C, K) indicated a significant increase between 5 and 10 μM curcumin-treated samples. The measurement of outer plexiform layer (OPL, I) did not show any significant differences among groups examined.*P < 0.05 and **P < 0.01 [n = 5]. Data were expressed as mean ± SEM. One-way ANOVA, Tukey’s post hoc test.

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