Fig. 7: Curcumin influences SUMO-1 and degradation pathway activity in ex vivo model of retinal degeneration. | Cell Death Discovery

Fig. 7: Curcumin influences SUMO-1 and degradation pathway activity in ex vivo model of retinal degeneration.

From: Retinal ganglion cell loss in an ex vivo mouse model of optic nerve cut is prevented by curcumin treatment

Fig. 7

A–D Representative western blots and relative quantifications of SUMO-1, UBC9, Ubiquitin, and LC3B. A Graphs showed an increase of SUMO-1ylated proteins and UBC9 level at time 24 in retinas homogenates if compared to time 0. The treatment with 10 μM of curcumin induced a significant augmentation of SUMO-1 as well as UBC9 levels, while 5 μM caused a slight reduction of SUMO-1 activity. B Histograms showed at time 24 a significant increase of Ubiquitin level and a reduction of LC3BII/I ratio induced by 5 and 10 μM curcumin administration. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 [n = 5]. Data were expressed as mean ± SEM. One-way ANOVA, Tukey’s post hoc test.

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