Fig. 2: PIAS1 promotes miR-124 expression through SUMOlation of PPAR-γ. | Cell Death Discovery

Fig. 2: PIAS1 promotes miR-124 expression through SUMOlation of PPAR-γ.

From: PIAS1 alleviates diabetic peripheral neuropathy through SUMOlation of PPAR-γ and miR-124-induced downregulation of EZH2/STAT3

Fig. 2

A The SUMOlation of PPAR-γ as detected by Co-IP. B The SUMOlation of PPAR-γ after PIAS1 was knocked down or treated with high glucose in SC4 cells as detected by Co-IP. C The interaction between PIAS1 and PPAR-γ as detected by Co-IP. D The significant co-expression of PIAS1 and PPAR-γ as detected by MEM analysis. E The binding of PPAR-γ with miR-124 promoter as detected by ChIP. F The binding of PPAR-γ with miR-124 promoter after PIAS1 knockdown as detected by ChIP. G The expression of PPAR-γ in cells after PPAR-γ knockdown or overexpression as determined by western blot assay. The one with the best knockdown efficiency was selected for subsequent experiments. H The expression of PPAR-γ in cells after PPAR-γ knockdown or overexpression as determined by RT-qPCR. I The expression of miR-124 in cells after PPAR-γ knockdown or overexpression as determined by RT-qPCR. J qRT-PCR measurement of miR-124 expression in cells treated with PPAR-γ or mutant PPAR-γ-K77R vectors. *p < 0.05. Data between two groups were compared by independent sample t test, and those among multiples by one-way ANOVA, followed by Tukey’s post hoc tests. The experiment was conducted in triplicates.

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