Fig. 3: PIAS1 promotes autophagy of Schwann cells via miR-124. | Cell Death Discovery

Fig. 3: PIAS1 promotes autophagy of Schwann cells via miR-124.

From: PIAS1 alleviates diabetic peripheral neuropathy through SUMOlation of PPAR-γ and miR-124-induced downregulation of EZH2/STAT3

Fig. 3

A The expression of miR-124 in sciatic nerve tissues of C57BL/6 mice and db/db mice as determined by RT-qPCR. B, C SC4 cells were treated with high glucose, and the expression of miR-124 was detected by RT-qPCR. D SC4 cells were treated with high glucose, and the expression of PIAS1 was detected by western blot assay. E The number of LC3-II granules as measured by immunofluorescence staining. F The proportion of apoptosis as detected by flow cytometry. G The expression of apoptosis and autophagy-related proteins as determined by western blot assay. *p < 0.05. Data between two groups were compared by independent sample t test, and those among multiples by one-way ANOVA, followed by Tukey’s post hoc tests. The experiment was conducted in triplicates.

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