Fig. 3: Intestinal autophagy in IBD was further enhanced by CRH. | Cell Death Discovery

Fig. 3: Intestinal autophagy in IBD was further enhanced by CRH.

From: Corticotropin releasing hormone promotes inflammatory bowel disease via inducing intestinal macrophage autophagy

Fig. 3

C57BL/6 mice were administered DSS (3%) for 6 days (and a control group was provided with water only for comparison). For the CRH treatment group, CRH (or vehicle only) was intraperitoneally injected at a dose of 50 μg/kg body weight from day 1 to day 6 using saline as the vehicle. A, B The levels of autophagy-related proteins, including Beclin-1, the LC3-II/I ratio, and p62/SQSTM1, were detected by western blot. In comparison with the DSS + vehicle group, mice in the DSS + CRH group exhibited significantly increased levels of Beclin-1 and the LC3-II/I ratio, but a decrease of p62 (n = 5 per group). **P < 0.01 vs. the control group; #P < 0.05 vs. the DSS + vehicle group; ##P < 0.01 vs. the DSS + vehicle group. C The left edge of the left colon was separated and fixed and then H&E staining was used for the detection of inflammatory infiltration (which was assessed using a histological score). An increase was observed in the number of LC3 dots and the number of macrophages (which were colocalized).

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