Fig. 5: Chloroquine attenuated CRH-induced colonic damage in IBD mice. | Cell Death Discovery

Fig. 5: Chloroquine attenuated CRH-induced colonic damage in IBD mice.

From: Corticotropin releasing hormone promotes inflammatory bowel disease via inducing intestinal macrophage autophagy

Fig. 5

C57BL/6 mice were administered DSS (3%) for 6 days (and a control group was provided with water only for comparison). For the CRH, chloroquine, and CRH-chloroquine groups, CRH (50 μg/kg body weight) and/or chloroquine (60 mg/kg body weight) was intraperitoneally administrated from day 1 through day 6 (using saline as a vehicle). Initial body weight, DAI score, and colon length were subsequently determined by two researchers blinded to the treatment groups. In addition, inflammation in the left colon was assessed by HE staining. A–C In comparison with the DSS + vehicle group, mice in the DSS + CRH group exhibited significant increases in body weight loss, DAI scores, and colon shortening. In contrast, coadministration of chloroquine largely attenuated the aggravation effects of CRH on body weight loss and DAI Score, but no significant change in colon length (n = 8 per group). **P < 0.01 vs. the control group; ##P < 0.01 vs. the DSS + vehicle group; $$P < 0.01 vs. the DSS + CRH group. D, E H&E staining was used to assess histological scores in the left colon. In comparison with the DSS + vehicle group, mice in the DSS + CRH group demonstrated an aggravation of inflammatory infiltration. In contrast, coadministration of chloroquine largely alleviated the impact of CRH (n = 8 per group). **P < 0.01 vs. the control group; #P < 0.05 vs. the DSS + vehicle group; $P < 0.05 vs. the DSS + CRH group.

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