Fig. 6: Chloroquine attenuated the increase in autophagy and number of macrophages in IBD mice. | Cell Death Discovery

Fig. 6: Chloroquine attenuated the increase in autophagy and number of macrophages in IBD mice.

From: Corticotropin releasing hormone promotes inflammatory bowel disease via inducing intestinal macrophage autophagy

Fig. 6

C57BL/6 mice were administered DSS (3%) for 6 days (and a control group was provided with water only for comparison). For the CRH and CRH-rapamycin groups, CRH (50 μg/kg body weight) and/or rapamycin (1.25 mg/kg body weight) was intraperitoneally injected from day 1 through day 6 using saline as a vehicle. The left edge of the colon was isolated and fixed, and immunofluorescence staining was used to assess the number of macrophages (LC3 dots) and the level of autophagy (F4/80 dots). In comparison with the DSS + vehicle group, mice in the DSS + CRH group demonstrated a significant increase in the numbers of LC3 dots and F4/80 dots. This increase was attenuated following chloroquine administration. Notably, LC3 dots colocalized obviously with the F4/80 dots.

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