Fig. 7: Chloroquine alleviated the CRH-induced enhancement of inflammation in BMDMs under the challenge of LPS. | Cell Death Discovery

Fig. 7: Chloroquine alleviated the CRH-induced enhancement of inflammation in BMDMs under the challenge of LPS.

From: Corticotropin releasing hormone promotes inflammatory bowel disease via inducing intestinal macrophage autophagy

Fig. 7

Murine BMDMs were isolated and subsequently stimulated with LPS (100 ng/mL), CRH (10−8 M), and/or chloroquine (10 μM). Real-time qPCR and ELISA were then used to assess the levels of proinflammatory factors. In comparison with the LPS + vehicle group, CRH administration further increased the levels of IL-1β, TNF-α, and IL-18 mRNA (A) and protein (B). (n = 6 per group.) **P < 0.01 vs. the control group; ##P < 0.01 vs. the chloroquine + vehicle group; $$P < 0.01 vs. the chloroquine + CRH group.

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