Fig. 3: Ferroptosis contributes to the inhibition of HSC activation. | Cell Death Discovery

Fig. 3: Ferroptosis contributes to the inhibition of HSC activation.

From: Berberine alleviates liver fibrosis through inducing ferrous redox to activate ROS-mediated hepatic stellate cells ferroptosis

Fig. 3

HSC-LX2 cells were treated with erastin (10 μM) and sorafenib (10 μM) with or without the indicated inhibitors (Z-VAD-FMK, 10 μM; Fer-1, 1 μM; necrostatin-1, 10 μM) for 24 h. A Cell viability was assayed by CCK-8. B Live cells and dead cells were assayed by FDA staining and PI staining, respectively. C–F ROS generation, cellular Fe2+, GSH depletion, and lipid peroxidation MDA production was assayed. G, H Vimentin and α-SMA protein levels were assayed. The results are expressed as mean ± SD. Data are representative of three independent experiments; n = 3–6 in every group; Scales: 100 μm. Representative photographs are shown. Compared with the control group, **P < 0.01; compared with the ferroptosis-inducer group, ##P < 0.01.

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