Fig. 5: Cytosolic mtDNA activated the STING pathway after GSDMD knockdown. | Cell Death Discovery

Fig. 5: Cytosolic mtDNA activated the STING pathway after GSDMD knockdown.

From: Mitochondrial DNA leakage exacerbates odontoblast inflammation through gasdermin D-mediated pyroptosis

Fig. 5

A In an interval 48 h, anti-GSDMD siRNA was transfected mDPC6T cells twice, after the second transfection mtDNA overexpressed. B The mRNA level of CXCL10 and IFN-β in mDPC6T cells was assessed by qPCR with or without GSDMD knockdown in response to 24 h of mtDNA transfection. C The protein expression of STING, P-TBK1/TBK1, P-IRF3/IRF3 was analyzed by western blotting with or without GSDMD knockdown in response to 24 h of mtDNA transfection. The data are the means ± standard deviations of 3 independent experiments (p < 0.05*, p < 0.01**, p < 0.001***).

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