Fig. 4: miR-135a inhibited cardiac hypertrophy by targeting TCF7.
From: LncRNA4930473A02Rik promotes cardiac hypertrophy by regulating TCF7 via sponging miR-135a in mice
A The expressions of miR-135a in cardiac hypertrophy models in vitro. B Expression level of miR-135a in cardiomyocytes transfected with scramble or miR-135a mimics. C Immunostaining of α-SMA in cardiomyocytes transfected with miR-135a mimics. Bar: 20 μm. D, E Western blot results of β-MHC and BNP protein expression in cardiomyocytes transfected with miR-135a mimics. F, G mRNA levels of β-MHC and BNP in cardiomyocytes transfected with miR-135a mimics. H The binding sites of miR-135a and TCF7 were determined using TargetScan software. I Western blot results of TCF7 protein expression by miR-135a mimics. J The mRNA level of TCF7 in cardiomyocytes by miR-135a mimics. K Luciferase reporter activities of chimeric vectors carrying the luciferase gene and a fragment of TCF7 3′-UTR containing the binding sites of miR-135a. *P < 0.05 vs. TCF7-wt group, n = 3. L Western blot results of c-Myc protein expression by miR-135a mimics. M The mRNA level of c-Myc in cardiomyocytes by miR-135a mimics. *P < 0.05 vs. Control group, #P < 0.05 vs. Ang-II group, @P < 0.05 vs. miR-135a group, n = 6.
