Fig. 4: SYT8 enhances proliferation and metastasis of pancreatic cancer cells by upregulating SIRT1.
From: SYT8 promotes pancreatic cancer progression via the TNNI2/ERRα/SIRT1 signaling pathway
A Densitometric quantification and representative western blot images showing SYT8 and SIRT1 protein expression levels in BxPC-3 and PANC-1 cells co-expressing different siRNA or overexpression vectors against SYT8 and SIRT1. Glyceraldehyde 3-phosphate dehydrogenase was used as a loading control. B Cell proliferation was measured by CCK-8 analysis in cells expressing the different constructs measured over 4 days post-transfection. C, D Representative images and quantification of total colonies formed in BxPC-3 or PANC-1 cells expressing different vectors. E–H Representative images and quantification of cell migration and invasion in BxPC-3 or PANC-1 cells were measured using a Transwell system 48 h post-transfection. Scale bar: 100 μm. Data are presented as mean ± SD with at least three independent experiments. *P < 0.05.
