Fig. 5: ERRα favors SYT8-induced SIRT1 expression and pancreatic cancer progression.
From: SYT8 promotes pancreatic cancer progression via the TNNI2/ERRα/SIRT1 signaling pathway
A Densitometric quantification and representative western blot images showing SYT8, SIRT1, and ERRα protein expression levels in PANC-1 cells transfected with control (NC) or SYT8 overexpression vectors treated with a vehicle or C29, an inhibitor against ERRα for 24 h. B, C Representative images and quantification from colony-formation analyses of PANC-1 cells overexpressing SYT8 in the presence and absence of the inhibitor. D–F Quantification and representative images from Transwell migration and invasion assays. Scale bar: 100 μm. G Representative images from western blots. Immunoprecipitates with ERRα-coated beads using cell lysates from HPDE, BxPC-3, and Panc-1 cells were subjected to electrophoresis, blotted onto membranes, and probed with antibodies to visualize SIRT1 and ERRα expression. Data are presented as mean ± SD with at least three independent experiments. *P < 0.05.
