Fig. 5: TRAP1 interacted with MIC60 and decreased MIC60 ubiquitination to increase MIC60 protein levels in rats’ heart tissue in extracellular acidosis. | Cell Death Discovery

Fig. 5: TRAP1 interacted with MIC60 and decreased MIC60 ubiquitination to increase MIC60 protein levels in rats’ heart tissue in extracellular acidosis.

From: TRAP1 inhibits MIC60 ubiquitination to mitigate the injury of cardiomyocytes and protect mitochondria in extracellular acidosis

Fig. 5

A Detection of the colocalization of MIC60 (green) and TRAP1 (red) using confocal microscopy in rat’s heart tissue. B Immunoblotting analysis of TRAP1 and MIC60 expression in a co-IP assay performed in protein lysate of rat’s heart tissue with anti-TRAP1 or anti-MIC60 Magnetic beads, respectively. C–D Western blot was used to determine the transfection efficiency of TRAP1 (C) and MIC60 (D) in rats’ heart tissue. E Western blotting was used to detect MIC60 protein levels regulated by TRAP1. F Western blot was used to detect MIC60 protein ubiquitination. Data were the means ± SD from three independent experiments. Group comparisons were performed by one-way analysis of variance followed by Tukey’s post hoc test. &P < 0.05 vs. Normal group. #P < 0.05 vs. ov-Con group. ##P < 0.01 vs. ov-Con group. *P < 0.05 vs. sh-Con group. **P < 0.01 vs. sh-Con group.

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