Fig. 3: miRNA-130a-3p suppresses CP, CM and CI in HCC cells. | Cell Death Discovery

Fig. 3: miRNA-130a-3p suppresses CP, CM and CI in HCC cells.

From: LncRNA LINC00667 aggravates the progression of hepatocellular carcinoma by regulating androgen receptor expression as a miRNA-130a-3p sponge

Fig. 3

A Using starBase, LncBase and miRBase, LINC00667 was targeted by 8 miRNAs. B Relative expression of candidate miRNAs in HCCLM3 and Huh-7 cells, miRNA-130a-3p was selected for further analyses. Data (n = 3) were analyzed by student’s t test. C and D Expression levels of miRNA-130a-3p in HCC and paired adjacent tissues were determined by RT-qPCR assay (n = 63, paired t test). E Kaplan-Meier OS analysis of HCC patients with downregulated and upregulated miRNA-130a-3p expression. F Pearson’s correlation coefficients of the relationship between LINC00667 and miRNA-130a-3p expression in HCC tissues. G and H Proliferative abilities of HCCLM3/Huh-7 cells transfected with mimics or mimics-NC were evaluated by CCK-8 and Edu assays, respectively. Scale bar, 100 μm. Data were analyzed by student’s t test. I Migratory capabilities of HCCLM3/Huh-7 cells transfected with mimics or mimic-NC were determined by wound healing assays. Scale bar, 100 μm. Data were analyzed by student’s t test. J Invasive capabilities of HCCLM3/Huh-7 cells transfected with mimics or mimic-NC were determined by transwell assays. Scale bar, 150 μm. Data, mean ± S.D. *, **, *** and **** = P < 0.05, <0.01, <0.001 and <0.0001, respectively.

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