Fig. 3: Downregulation of UBQLN4 inhibits CRC cell proliferation, migration, and invasion in vitro. | Cell Death Discovery

Fig. 3: Downregulation of UBQLN4 inhibits CRC cell proliferation, migration, and invasion in vitro.

From: UBQLN4 is activated by C/EBPβ and exerts oncogenic effects on colorectal cancer via the Wnt/β-catenin signaling pathway

Fig. 3

A, B Knockdown of UBQLN4 by transfecting si-UBQLN4 in both HCT-8 and SW480 cells. The efficiency was detected using RT-qPCR (A) and western blot (B). The MTS experiment indicated that UBQLN4 knockdown inhibited the growth of both HCT-8 (C) and SW480 cells (D). The plate colony formation experiment was also used to detect the effect of UBQLN4 knockdown on the proliferation of HCT-8 (E) and SW480 cells (F). Transwell experiment of the migration and invasion of UBQLN4 knockdown HCT-8 (G) and SW480 cells (H). 200×, Scale bar: 200 μm. Wound healing experiment showing the effect of UBQLN4 knockdown on HCT-8 (I) and SW480 cell (J) migration. 100×, Scale bar: 200 μm. Schematic of the CytoSelect™ 24-Well Cell Migration Assay (K) and respective quantification of HCT-8 or SW480 cell migration and invasion after the downregulation of UBQLN4 (L). All data from at least three independent experiments are presented as the means ± SD. *p < 0.05, **p < 0.01, *** or ****p < 0.001.

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