Fig. 4: C/EBPβ induces UBQLN4 transcription in CRC cells. | Cell Death Discovery

Fig. 4: C/EBPβ induces UBQLN4 transcription in CRC cells.

From: UBQLN4 is activated by C/EBPβ and exerts oncogenic effects on colorectal cancer via the Wnt/β-catenin signaling pathway

Fig. 4

A Schematic illustration of pGL3-2000, pGL3-1000, pGL3-500, pGL3-250, pGL3-125, and pGL3-Basic. B, C Dual luciferase reporter assay was utilized to detect the core promoter region of UBQLN4. Overexpression of Ets-1 and C/EBPβ increased the luciferase activity of pGL3-250 in HCT-8 (D) and SW480 cells (E). RT-qPCR experiment showed that C/EBPβ upregulated UBQLN4 expression in HCT-8 (F) and SW480 cells (G). H Western blot experiment indicated that C/EBPβ increased UBQLN4 protein expression in both HCT-8 and SW480 cells. I Schematic diagram of the binding site for C/EBPβ in the UBQLN4 promoter region. J–L ChIP-qPCR showed greater enrichment of promoter amplicons of UBQLN4 in the anti-C/EBPβ group than in the IgG group of both HCT-8 and SW480 cells. The MTS experiment indicated that overexpression of C/EBPβ promoted the growth of both HCT-8 (M) and SW480 cells (N). All data from at least three independent experiments are presented as the means ± SD. ns not significant, *p < 0.05, **p < 0.01, *** or ****p < 0.001.

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