Fig. 6: UBQLN4-mediated effects are abolished by the downregulation of c-Myc. | Cell Death Discovery

Fig. 6: UBQLN4-mediated effects are abolished by the downregulation of c-Myc.

From: UBQLN4 is activated by C/EBPβ and exerts oncogenic effects on colorectal cancer via the Wnt/β-catenin signaling pathway

Fig. 6

Plate colony formation experiment showed that silencing c-Myc expression in both HCT-8 (A, B) and SW480 cells (C, D) reversed the increased proliferation induced by UBQLN4 overexpression. The MTS experiment also showed that silencing c-Myc expression in both HCT-8 (E) and SW480 cells (F) reversed the increased proliferation induced by UBQLN4 overexpression. G–L Transwell and CytoSelect™ 24-Well Cell Migration assays indicated that silencing c-Myc expression in both HCT-8 and SW480 cells abolished the increased migration and invasion induced by upregulation of UBQLN4, 100×, Scale bar: 400 μm. M Western blot showed that silencing c-Myc expression in both HCT-8 and SW480 cells abolished the increased expression of c-Myc induced by the upregulation of UBQLN4. All data from at least three independent experiments are presented as the means ± SD. *p < 0.05, **p < 0.01, *** or ****p < 0.001.

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