Fig. 3: Knockdown of LINC00205 inhibited cell proliferation and tumor growth.

A Cell proliferation was measured by IncuCyte live cell analysis system. B The activity of DNA replication was detected by EdU staining. The representative images were shown (left) and the quantitative measurements of the percentage of EdU positive cells were presented in column shows (right). n = 6 independent experiments, *P < 0.05 vs shControl. Scale bar, 200 μm. C Colony formation assay. D Cell cycle analysis of the indicated groups. Figure shown was a representative experiments (up). The percentage of cells in each phase was calculated (down). The BGC823 cells with stable knockdown of LINC00205 were xenograft subcutaneously into the immune-deficient nude mice (n = 5 for each group). The tumor volumes were calculated and recorded when the xenograft models established. E The tumor growth curves. *P < 0.05 vs shControl. At the end of experiment, the mice were sacrificed and the tumors were collected. F The representative images of xenograft tumors (left). Tumor weight was statistically plotted (right).