Fig. 5: Overexpressed TGF-βRII inhibited PKM2 nuclear translocation to glycolysis. | Cell Death Discovery

Fig. 5: Overexpressed TGF-βRII inhibited PKM2 nuclear translocation to glycolysis.

From: TGF-βRII regulates glucose metabolism in oral cancer-associated fibroblasts via promoting PKM2 nuclear translocation

Fig. 5

A, B In protein level, PKM2 in the cytoplasm displayed with no obvious change among the groups. C, D Overexpression of TGF-βRII decreased PKM2 level in the nucleus of CAFsTGF-βRII with a significant statistical difference. E, F Immunoblot showed an augmentation of phosphorylated ERK1/2 in CAFsTGF-βRII while no obvious change of non-phosphorylated ERK1/2. G, H In CAFsTGF-βRII cells, the specific inhibitor of p-ERK1/2 (U0126) suppressed its phosphorylation and inhibited the PKM2 level in the nucleus simultaneously. I–K Immunoblot showed a reduction of GLUT1 and LDHA decreased in CAFsTGF-βRII with a statistical difference both in the protein and mRNA level while CCND1 exhibited no obvious change. Relative mRNA or protein expression level was quantified after normalization to β-tubulin or PCNA. n = 3 independent experiments; error bars, mean ± SD; n.s, not significant, **P < 0.01, ***P < 0.001; t test.

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