Fig. 2: FAM225A promotes GC cell proliferation, migration, and invasion and induces cell apoptosis. | Cell Death Discovery

Fig. 2: FAM225A promotes GC cell proliferation, migration, and invasion and induces cell apoptosis.

From: Long noncoding RNA FAM225A promotes the malignant progression of gastric cancer through the miR-326/PADI2 axis

Fig. 2

A–D Measuring MKN45 or AGS cell proliferation ability when knockdown or overexpression of FAM225A through colony formation assays and the Edu assays (scale bar: 100 μm for Edu assay). E, F Flow cytometric analysis of MKN45 or AGS cells by downregulating or upregulating FAM225A expression to detect the cell apoptosis. G–J Wound healing assay and Transwell assay revealed that silencing FAM225A could dramatically attenuate MKN45 cells migration ability, while overexpression of FAM225A has the opposite effects in AGS cells (scale bar: 200 μm for transwell assay, 100 μm for wound healing assay). K, L Western blot analysis of the EMT-related molecules in GC cells when knockdown or overexpression of FAM225A. p values were obtained by Student’s t test. Data are presented as means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001.

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