Fig. 6: FAM225A modulates PADI2 expression by competitively binding miR-326. | Cell Death Discovery

Fig. 6: FAM225A modulates PADI2 expression by competitively binding miR-326.

From: Long noncoding RNA FAM225A promotes the malignant progression of gastric cancer through the miR-326/PADI2 axis

Fig. 6

A, B Relative expression of PADI2 in GC tissues according to TCGA database and our own 68 GC tissues. C–F Detecting PADI2 mRNA and protein expression when knockdown or overexpression of miR-326 in MKN45 or AGS cells by using qRT-PCR and western blot. G Predicting binding sites between PADI2 and miR-326. H, I The relative luciferase activity of the wild-type PADI2 was reduced by upregulating miR-326. J–M Detecting PADI2 mRNA and protein expression when MKN45 cells transfected with siNC + Anti-NC, Anti-NC + siFAM225A, siFAM225A + miR-326 inhibitor, or when AGS cells transfected with Vector + miR-NC, miR-NC + FAM225A, FAM225A + miR-326 mimics by using qRT-PCR and western blot. N, O The correlation between the expression of PADI2 and the expression of miR-326 or FAM225A was analyzed in 68 GC tissues. p values were obtained by Student’s t test. Data are presented as means ± SD. FC fold change, **p < 0.01, ***p < 0.001.

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