Fig. 2: DDX39B promotes the proliferation of colorectal cancer in vitro.
From: DDX39B contributes to the proliferation of colorectal cancer through direct binding to CDK6/CCND1
A Transfected efficiency was determined using western blotting in HCT116, SW480, and RKO cells with overexpressed or silenced DDX39B. B CCK8 assay was used for exploring the effect of overexpression of DDX39B on the proliferation of colorectal cancer in HCT116, SW480 cell lines; and the effect of inhibition of DDX39B in SW480, RKO cell lines (one-sample t-test). C Colony-forming assay was used for detecting the effect of overexpression and inhibition of DDX39B on the proliferation of colorectal cancer in HCT116, SW480 and RKO cell lines, 6-well plate, 1000 cells per well. The number of colonies were calculated in the form of a bar chart, by (one-sample t-test) pairwise. D Flow cytometry was used to detect the cell cycle of RKO/Scramble, RKO/shDDX39B; SW480/Scramble, SW480/shDDX39B; HCT116/Vector, HCT116/DDX39Boe; SW480/Vector, SW480/DDX39Boe groups with PI staining. Bar graph were used to show the G1 phase of cell cycle (one-sample t-test). The experiments were performed in three times.
