Fig. 5: DDX39B promotes the cell proliferation of colorectal cancer via targeting the CDK6/CCND1.
From: DDX39B contributes to the proliferation of colorectal cancer through direct binding to CDK6/CCND1
A RIP-seq of SW480 and HCT116/DDX39Boe showed that DDX39B binds directly to multiple sites of CDK6 and CCND1 pre-mRNA (red box: a partial sequence of the exon1, validated by RIP). B, C RIP-PCR assay was used to validate the binding of DDX39B to the CDK6/CCND1. D RT-PCR and agarose gel electrophoresis assay showed the splicing effect of DDX39B on CDK6/CCND1 pre-mRNA. E The relative mRNA expression levels of CDK6 and CCND1 gene in DDX39B ectopic overexpression siCDK6/CCND1 HCT116 cell line and its control by the real-time PCR (one-sample t-test). F CDK6 and CCND1 protein expression levels of DDX39B ectopic overexpression siCDK6/CCND1 HCT116 cell line and its control. G CCK8 assay on DDX39B ectopic overexpression siCDK6/CCND1 HCT116 cell line and its control, by one-sample t-test. H Colony formation assay on DDX39B ectopic overexpression siCDK6/CCND1 HCT116 cell line and its control, 12-well plate, 400 cells per well. The bar chart demonstrated the analysis of colony numbers by one-sample t-test.
