Fig. 5: Single-cell transcriptional analysis unveils functional heterogeneity of T cells and NK cells in patients with FHP. | Cell Death Discovery

Fig. 5: Single-cell transcriptional analysis unveils functional heterogeneity of T cells and NK cells in patients with FHP.

From: Characterizing cellular heterogeneity in fibrotic hypersensitivity pneumonitis by single-cell transcriptional analysis

Fig. 5

A t-SNE visualization of effector memory CD8+ T cells, NK cells, T regulatory cells, KLRG1+ CD8+ terminal effector T cells, Th1/Th17 cells, and Vd2 gamma delta T cells. B AUCell analysis of the relative gene set enrichment scores in Pro-inflammation signature. The t-SNE map showing AUC scores of selected gene signature (left); the box plot showing AUC scores of selected gene signature in each cell type (right). C–F AUCell analyses of the relative gene set enrichment scores in TCR Signaling signature (C), Cytotoxicity signature (D), Chemokine signature (E), and TNF Family Receptors signature (F). G t-SNE visualization of TCR clone type. Cells are colored based on clone size (left), TCR chains (middle), or clone frequency (right). H TBX21 motif showed greater enrichment of regulon activity for NK cells and terminal effector CD8+ T cells using SCENIC analysis (left); STAT1 motif showed greater enrichment of regulon activity for Vd2 gamma delta T cells using SCENIC analysis (right). I t-SNE visualization of AUC values of TBX21 (left) or STAT1 (right) in T cells and NK cells. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

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