Fig. 4: LINC00858 promoted the malignant phenotype of colon cancer cells and inhibited their apoptosis by regulating the RAD21-PCNP axis. | Cell Death Discovery

Fig. 4: LINC00858 promoted the malignant phenotype of colon cancer cells and inhibited their apoptosis by regulating the RAD21-PCNP axis.

From: LINC00858 promotes colon cancer progression through activation of STAT3/5 signaling by recruiting transcription factor RAD21 to upregulate PCNP

Fig. 4

A The expression of LINC00858 in HCT166 and SW480 cells determined by RT-qPCR. B The expression of RAD21 in HCT166 and SW480 cells determined by RT-qPCR. C PCNP and RAD21 protein expression in HCT166 and SW480 cells in each group determined by Western blot assay. D Expression of PCNP in HCT166 and SW480 cells determined by RT-qPCR. E Proliferation of HCT166 and SW480 cells in each group determined by CCK-8 assay. F Apoptosis of HCT166 and SW480 cells in each group detected by flow cytometry. G Protein expression of cleaved caspase-3/total Caspase 3 and cleaved PARP/total PARP in HCT166 and SW480 cells in each group determined by Western blot assay. H The migration and invasion of HCT166 and SW480 cells examined by Transwell assay. *p < 0.05. Data from multiple groups were compared using one-way ANOVA with Tukey’s post hoc tests. Repeated measures ANOVA with Bonferroni’s post hoc test was used to compare data from multiple time points,. All cell experiments were repeated three times.

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