Fig. 5: PCNP activates the STAT3/5 signaling pathway to promote colon cancer cell proliferation, migration and invasion while inhibiting apoptosis. | Cell Death Discovery

Fig. 5: PCNP activates the STAT3/5 signaling pathway to promote colon cancer cell proliferation, migration and invasion while inhibiting apoptosis.

From: LINC00858 promotes colon cancer progression through activation of STAT3/5 signaling by recruiting transcription factor RAD21 to upregulate PCNP

Fig. 5

A The expression of PCNP in HCT166 and SW480 cells was determined by RT-qPCR. B The expression of PCNP, STAT3 and STAT5, and phosphorylation levels of STAT3 and STAT5 in HCT166 and SW480 cells detected by Western blot assay. C HCT166 and SW480 cell proliferation assessed by CCK-8 assay. D HCT166 and SW480 cell apoptosis detected by flow cytometry. E HCT166 and SW480 cell apoptosis detected by TUNEL staining. F HCT166 and SW480 cell invasion assessed by Transwell assay. G The expression of cleaved caspase-3/total Caspase 3 and cleaved PARP/total PARP in HCT166 and SW480 cells detected by Western blot assay. *p < 0.05. Data from multiple groups were compared using one-way ANOVA with Tukey’s post hoc tests. Repeated measures ANOVA combined with Bonferroni’s post hoc test was used to compare data from different time points,. All cell experiments were repeated three times.

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