Table 1 List of primers used for quantitative RT-PCR.

From: Role of mTORC1 activity during early retinal development and lamination in human-induced pluripotent stem cell‐derived retinal organoids

Gene

Primer sequence

Forward

Reverse

Pax6

5′-CGG AGT GAA TCA GCT CGG TG-3

5′-CCG CTT ATA CTG GGC TAT TTT GC-3

AtoH7

5′-GTG GGG CCA GGA TAA AAA GC-3′

5′-CGG GTC AGA GCC ATG TAG4-3′

Islet-1

5′-GCG GAG TGT AAT CAG TAT TTG GA-3′

5′-GCA TTT GAT CCC GTA CAA CCT-3′

Brn3b

5′-TGA CAC ATG AGC GCT CTC ACT TAC-3′

5′-ACC AAG TGG CAA ATG CAC CTA-3′

Tuj1

5′-GGC CAA GGG TCA CTA CAC G-3′

5′-GCA GTC GCA GTT TTC ACA CTC-3′

Rictor

5′-GGAAGCCTGTTGATGGTGAT-3′

5′-GGCAGCCTGTTTTATGGTGT-3′

Raptor

5′-ACTGATGGAGTCCGAAATGC-3′

5′-TCATCCGATCCTTCATCCTC-3′

mTOR

5′-ACA GAG TTG GAG CAC AGT GG-3′

5′-GGC CAC TAA CCT GTG CCA AT-3′

S6K1

5′-GCACAGCAAATCCTCAGACA-3′

5′-GATGCTTCCCCACTCATTGT-3

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