Fig. 4: YAP stimulates PPP by activating 6PGL. | Cell Death Discovery

Fig. 4: YAP stimulates PPP by activating 6PGL.

From: YAP ISGylation increases its stability and promotes its positive regulation on PPP by stimulating 6PGL transcription

Fig. 4

A Schematic presentation of PPP from glucose to Rib-5-P. B, C NADPH (B) and Rib-5-P (C) concentration in control, YAP overexpression or knockout A549 and H1299 cells. D, E Indicated mRNA levels in control, YAP overexpression or knockout A549 (D) and H1299 (E) cells. F 6PGL and YAP protein levels in control, YAP overexpression or knockout A549 and H1299 cells. G–J NADPH (G), Rib-5-P concentration (H), and colony formation (I, J) were measured in WT or YAP−/− A549 and H1299 cells with or without 6PGL overexpression. Scale bar, 200 μm. K, L NADPH (K) and Rib-5-P concentration (L) were measured in WT or 6PGL−/− A549 and H1299 cells with or without YAP overexpression. The data are shown as the mean ± SD from three biological replicates (including IB). Data in B–E, G–I, K, L were analyzed using a one-way ANOVA test. **P < 0.01, NS nonsignificant.

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