Fig. 4: SP600125 inhibited GSDME-mediated pyroptosis in HK cells. | Cell Death Discovery

Fig. 4: SP600125 inhibited GSDME-mediated pyroptosis in HK cells.

From: Blocking GSDME-mediated pyroptosis in renal tubular epithelial cells alleviates disease activity in lupus mice

Fig. 4

HK2 cells were pretreated with SP600125 for 1 h and then exposed to TNF-α plus CHX for 12 h. A The levels of LDH were measured. B Phase-contrast images of HK2 cells were collected. C HK2 cells were stained with Hoechst (blue) and PI (red). D Immunofluorescence staining of GSDME (red). E The expression of GSDME-FL and GSDME-N was measured by Western blotting. F The sera of lupus patients (n = 5) or healthy controls (n = 3) were mixed together and then added to the medium to prepare 10% cell culture medium. HK2 cells were pretreated with SP600125 for 1 h and then exposed to the serum of lupus patients or normal controls, followed by Western blot and quantitative analysis of the expression of GSDME. The data are shown as the mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001.

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