Fig. 5: The relationship of MIF, NF-κB and pyroptosis in HK-2 cells of vector+PBS, vector+LPS, knockdown MIF + PBS and knockdown MIF + LPS group. | Cell Death Discovery

Fig. 5: The relationship of MIF, NF-κB and pyroptosis in HK-2 cells of vector+PBS, vector+LPS, knockdown MIF + PBS and knockdown MIF + LPS group.

From: Downregulation of macrophage migration inhibitory factor attenuates NLRP3 inflammasome mediated pyroptosis in sepsis-induced AKI

Fig. 5

A MIF, pyroptosis related proteins (NLRP3, ASC, Caspase-1, Caspase-1 p20, Gasdermin D and its N-terminal fragment), p65 and phosphorylated p65 were detected, and their expressions relative to GAPDH were shown. B HK-2 cells were stained via immunofluorescence. MIF was tagged by FITC (green) and NLRP3 was tagged by Cy3 (red). The white arrow pointed to the overlapped area of high fluorescence. Scale bar = 100 μm. C Cell viability of HK-2 cells was evaluated via CCK-8 method. D HK-2 cells were stained by annexin-V-FITC and propidium iodide(PI). E PI stained HK-2 cells were counted by flow cytometry. Data were mean ± SD from three independent experiments. Each point represented an independent data. Group comparisons were performed by (A) & (E) one-way ANOVA followed by Tukey’s post hoc test, and (C) two-way ANOVA followed by Tukey’s post hoc test. (N = 3/group, #P < 0.05 vs vector + PBS group, *P < 0.05 vs vector + LPS group, NS means no significant difference).

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