Fig. 3: Knockout of circRBM33 reduces glycolysis and promotes apoptosis of BCa cells. | Cell Death Discovery

Fig. 3: Knockout of circRBM33 reduces glycolysis and promotes apoptosis of BCa cells.

From: CircRBM33 downregulation inhibits hypoxia-induced glycolysis and promotes apoptosis of breast cancer cells via a microRNA-542-3p/HIF-1α axis

Fig. 3

A CircRBM33 expression in MCF-7 cells after knocking down circRBM33; B Glucose consumption in MCF-7 cells after knocking down circRBM33; C Lactate production in MCF-7 cells after knocking down circRBM33; D Western blot detected HK2, LDHA and GLUT1 expression in MCF-7 cells after knocking down circRBM33; E CCK-8 detected MCF-7 cell proliferation after knocking down circRBM33; F Flow cytometry detected MCF-7 cell apoptosis after knocking down circRBM33 (Under normoxia, the apoptotic rate increased from 8.15% to 17.25% after knocking down circRBM33; Under hypoxia, the apoptotic rate increased from 2.58% to 8.95% after knocking down circRBM33); *P < 0.05. Data were expressed in the form of mean ± standard deviation (Repetitions = 3). The comparison between two groups should be performed by t test.

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