Fig. 7: Overexpression of HIF-1α reverses the inhibitory effect of upregulated miR-542-3p on glycolysis and apoptosis of BCa cells. | Cell Death Discovery

Fig. 7: Overexpression of HIF-1α reverses the inhibitory effect of upregulated miR-542-3p on glycolysis and apoptosis of BCa cells.

From: CircRBM33 downregulation inhibits hypoxia-induced glycolysis and promotes apoptosis of breast cancer cells via a microRNA-542-3p/HIF-1α axis

Fig. 7

A, B HIF-1α expression in MCF-7 cells after upregulating miR-542-3p and HIF-1α; C Glucose consumption in MCF-7 cells after upregulating miR-542-3p and HIF-1α; D Lactate production in MCF-7 cells after upregulating miR-542-3p and HIF-1α; E Western blot detected HK2, LDHA and GLUT1 expression in MCF-7 cells after upregulating miR-542-3p and HIF-1α; F CCK-8 detected MCF-7 cell proliferation after upregulating miR-542-3p and HIF-1α; G Flow cytometry detected MCF-7 cell apoptosis after upregulating miR-542-3p and HIF-1α (Under normoxia, after upregulating miR-542-3p, the apoptotic rate increased from 7.28% to 16.88%, and after upregulating miR-542-3p and HIF-1α, the apoptotic rate decreased from 17.25% to 9.14%; Under hypoxia, after upregulating miR-542-3p, the apoptotic rate increased from 3.20% to 7.88%, and after upregulating miR-542-3p and HIF-1α, the apoptotic rate decreased from 8.50% to 3.340%); *P < 0.05. Data were expressed in the form of mean ± standard deviation (Repetitions = 3). The comparison among multiple groups should be performed by one-way ANOVA, while pairwise comparison was by Tukey’s method.

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