Fig. 7: The Sirt5-OPTN K108su axis improves RGCs function in STZ-induced diabetic rats.
A, B Stimulus intensity responses for the STR, showing the pSTR and nSTR in rats whose eyes were injected with OPTN-WT, OPTN-K108R or scrambled lentivirus. Representative images (A) and quantitative data (B) are provided. Absolute amplitude appears on the y-axis, and the 4 flash intensities (log cds/m2) are on the x-axis. A description of the data points appears at the bottom of the graph. Green *p < 0.05 between LV.OPTNK108R- and LV.OPTNWT-injected groups; black *p < 0.05 between DM and NM retinas subjected to LV.Flag. C, D pSTR and nSTR in rats subjected to eye injection of LV.Sirt5 and OPTN-WT, OPTN-K108E or scrambled lentivirus. Representative images (C) and quantitative data (D) are provided. Green* indicates p < 0.05 between the LV.Sirt5 + OPTNK108E- and LV.Sirt5 + OPTNWT-injected groups. Black* indicates p < 0.05 between the LV-Sirt5+Flag and scrambled LV.HA + Flag groups. E, F PERG in rats subjected to eye injection of OPTN-WT, OPTN-K108R or scrambled lentivirus. Representative images (E) and quantitative data (F) are provided. G, H PERG in rats subjected to eye injection of LV.Sirt5 and OPTN-WT, OPTN-K108E or scrambled lentivirus. Representative images (G) and quantitative data (H) are provided. The data are shown as the mean ± SD (n = 6). *p < 0.05. **p < 0.01. STR scotopic threshold response, pSTR/nSTR positive STR and negative STR, PERG pattern electroretinogram.
