Fig. 7: miR-21-5p inhibits zymophagy by regulating Trim33.

A Co-localization analysis of endogenous ubiquitin and zymogen particles. B The photos of the activation of each group of zymogen particles were taken by a confocal microscope. C Flow cytometry to detect the fluorescence intensity value of each group. D Detect the activation level of zymogen in each group. E–H The mRNA and protein levels of VMP1,Trim33 and P62 in each group were detected by RT-PCR and Western blot (t-test, ***p < 0.001, **p < 0.01, *p < 0.05).