Fig. 7: GOLPH3 facilitates autophagy via the suppression of phosphorylation of Akt at Ser473. | Cell Death Discovery

Fig. 7: GOLPH3 facilitates autophagy via the suppression of phosphorylation of Akt at Ser473.

From: Golgi phosphoprotein 3 induces autophagy and epithelial–mesenchymal transition to promote metastasis in colon cancer

Fig. 7

A KEGG (Kyoto Encyclopedia of Genes and Genomes) analyses differentially expressed genes between the HCT-116-PMSCE-Vector and HCT-116-PMSCV-GOLPH3 cells. B Western blot analysis of GOLPH3-overexpressing and control HCT-116 and HT-29 cell lines. Akt Ser473 phosphorylation was decreased in GOLPH3-overexpressing cells. In GOLPH3-silenced colorectal cancer cells, Akt Ser473 phosphorylation was enhanced. C MK-2206 2HCl was used to repress Akt phosphorylation. P62 and E-cadherin were stimulated, and N-cadherin was repressed when GOLPH3 was silenced. When CRC cell lines were cultured in Akt Ser473 phosphorylation inhibitor, P62 and E-cadherin were repressed, and N-cadherin was enhanced. GAPDH was used as an internal reference. D The above results demonstrated that GOLPH3 induces autophagy and EMT to promote metastasis in colon cancer, via the repression of Akt Ser473 phosphorylation.

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