Fig. 4: FBXW5 regulates the Hippo signaling pathway. | Cell Death Discovery

Fig. 4: FBXW5 regulates the Hippo signaling pathway.

From: The E3 ubiquitin ligase, FBXW5, promotes the migration and invasion of gastric cancer through the dysregulation of the Hippo pathway

Fig. 4

A The association between the expression of FBXW5 and CTGF, CYR61, and c-Myc is plotted on the GEPIA website. B Quantitative real-time PCR was performed using the HGC-27 cell line to determine the effect of FBXW5 knockdown on the Hippo pathway target genes at the mRNA level. Data were presented as mean ± SEM from three biologically independent experiments. C Western blots were used to determine the effect of FBXW5 knockdown on the Hippo pathway protein. D Immunofluorescence results for the YAP1 protein after the knockdown or upregulation of FBXW5 in GC cells. Bar scale: 50 μm. E The association between YAP1 and FBXW5 was evaluated via IHC using 45 paraffin-embedded GC samples. Bar scale: 500 μm (20×), 50 μm (200×).

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