Fig. 5: FBXW5 binds to LATS1 and promotes its degradation. | Cell Death Discovery

Fig. 5: FBXW5 binds to LATS1 and promotes its degradation.

From: The E3 ubiquitin ligase, FBXW5, promotes the migration and invasion of gastric cancer through the dysregulation of the Hippo pathway

Fig. 5

A, B Co-immunoprecipitation assays were carried out using HEK-293T cells after transfection with myc-FBXW5, flag-LATS1, flag-LATS2, or flag-YAP1 plasmid. C Co-immunoprecipitation assays were carried out using HEK-293T cells after transfection with myc-FBXW5ΔF-box (ΔF), ΔWD, and flag-LATS1. D After cells were treated with 20 μg/ml cycloheximide (CHX) for distinct lengths of time, western blot analysis was conducted. E Cells were treated with the proteasome inhibitor, MG132, and the protein level of LATS1 was determined using western blot analysis. F BGC-823 cells overexpressing FBXW5 were treated with MG132 for 6 h and analyzed to determine LATS1 expression. G, H In vivo protein ubiquitination assays were carried out using the GC cell lines, HGC-27 and BGC-823.

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