Fig. 6: Silencing of FBXW5 attenuates the growth of tumor xenografts. | Cell Death Discovery

Fig. 6: Silencing of FBXW5 attenuates the growth of tumor xenografts.

From: The E3 ubiquitin ligase, FBXW5, promotes the migration and invasion of gastric cancer through the dysregulation of the Hippo pathway

Fig. 6

A Western blot verification of the efficiencies of lentivirus transfection. B Xenograft tumors were harvested after monitoring for 20 days, and growth curves were plotted using xenograft tumors derived from the LV-scramble-shRNA group or LV-shFBXW5 group (mean ± SD, n = 6). C IHC was carried out and the results were quantified using the grading standards described above (mean ± SEM, n = 6). D IHC was conducted to determine the expression of FBXW5 and YAP1, and the results were quantified using the grading standards described above (mean ± SEM, n = 6). E Western blotting was used to detect the FBXW5 and CTGF proteins in six xenograft tumors. The protein levels of FBXW5 and CTGF were quantified and normalized to the level of β-actin. F The mRNA levels of CTGF were detected in six xenograft tumors using qPCR (mean ± SEM, n = 6). *P < 0.05, **P < 0.01, ***P < 0.001.

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