Fig. 3: Ketamine protected mice from TNF-α-induced cecal damage by suppressing the phosphorylation of RIP3 and MLKL. | Cell Death Discovery

Fig. 3: Ketamine protected mice from TNF-α-induced cecal damage by suppressing the phosphorylation of RIP3 and MLKL.

From: Ketamine inhibits TNF-α-induced cecal damage by enhancing RIP1 ubiquitination to attenuate lethal SIRS

Fig. 3

A Representative images of cecal tissue from mice in each group at 12 h after the injection of 15 μg of TNF-α. B When a mouse became moribund, tissues were collected, sectioned, and stained with H&E. Representative images are shown. Scale bar, 100 μm. TNF-induced cecal damage at the indicated time was scored and is shown in C. D, E Immunofluorescent staining and statistical analysis of the expression of the necroptosis markers p-MLKL and p-RIP3 in cecal tissue from mice in each group at 12 h after the injection pf 15 μg of TNF-α. F Western blot analysis of the expression of the necroptosis markers MLKL, p-MLKL in cecal tissue from mice in each group at 12 h after the injection pf 15 μg of TNF-α. All data are shown as the mean ± SEM. n = 4/group.

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