Fig. 4: Upregulation of WWP1 by YTHDF1 inhibits activation of NLRP3 inflammasomes and caspase-1-mediated cleavage of GSDMD. | Cell Death Discovery

Fig. 4: Upregulation of WWP1 by YTHDF1 inhibits activation of NLRP3 inflammasomes and caspase-1-mediated cleavage of GSDMD.

From: YTHDF1 alleviates sepsis by upregulating WWP1 to induce NLRP3 ubiquitination and inhibit caspase-1-dependent pyroptosis

Fig. 4

A The expression of YTHDF1 and WWP1 in cells with different treatment assessed by RT-qPCR. B The expression of TNF-α and IL-1β determined by ELISA. C LDH level in cells with different treatment. D The activation of NLRP3 inflammasomes measured by Western blot assay. E Protein levels of GasD-N and GasD-FL detected by Western blot assay. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. ‘ns’ indicates no significant difference. Cell experiments were repeated 3 times.

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