Fig. 5: Effect of KIAA1429 on regulating the expression of SIRT1. | Cell Death Discovery

Fig. 5: Effect of KIAA1429 on regulating the expression of SIRT1.

From: m6A methyltransferase KIAA1429 acts as an oncogenic factor in colorectal cancer by regulating SIRT1 in an m6A-dependent manner

Fig. 5

A, B SW480 and HCT116 cells were infected with scr and KIAA1429 shRNA, the mRNA and protein level of SIRTs were detected by QRT-PCR (A) and western blotting (B). **P < 0.01. C, D SW480 and HCT116 cells were transfected with empty vector (EV) and KIAA1429 plasmid, the mRNA and protein level of SIRTs were detected by QRT-PCR (C) and western blotting (D). **P < 0.01. E The proliferation of SW480 and HCT116 cells after KIAA1429 depletion followed with or without SIRT1 overexpression was measured using CCK- 8 assay. *P < 0.05. F The growth of SW480 and HCT116 cells over 14 days after KIAA1429 depletion followed with or without SIRT1 overexpression was measured using colony formation assay.

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