Fig. 2: WWP1 silencing suppressed proliferation, migration, and invasion of HuCCT1 cells in vitro. | Cell Death Discovery

Fig. 2: WWP1 silencing suppressed proliferation, migration, and invasion of HuCCT1 cells in vitro.

From: WWP1 upregulation predicts poor prognosis and promotes tumor progression by regulating ubiquitination of NDFIP1 in intrahepatic cholangiocarcinoma

Fig. 2

A WWP1 protein levels in the four ICC cell lines and normal human intrahepatic biliary epithelial cell (HIBEC) were determined by western blotting. B The efficiency of WWP1 knockdown was estimated by western blotting. C CCK-8 assay, D colony formation assay, E EdU assay were performed to estimate the proliferation of HuCCT1 cells after WWP1 silencing, n = 3. Overexpression of WWP1 again in HuCCT1 cells infected with the lentiviral shWWP1 rescued the ability of proliferation (C, D). F, G Transwell assays were performed to detect the migration (F) and invasion (G) of HuCCT1 cells after WWP1 silencing, n = 3. **P < 0.01, ***P < 0.001.

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