Fig. 6: WWP1 interacted with NDFIP1. | Cell Death Discovery

Fig. 6: WWP1 interacted with NDFIP1.

From: WWP1 upregulation predicts poor prognosis and promotes tumor progression by regulating ubiquitination of NDFIP1 in intrahepatic cholangiocarcinoma

Fig. 6

A Proteins that interacted with WWP1 were detected by Coomassie blue staining SDS-PAGE gel and mass spectrometry. NDFIP1 was identified as its molecular weight located on one specific band (red arrow). B Gene Ontology (GO) analysis of WWP1-binding proteins identified by mass spectrometry was performed. C WWP1 binding proteins were analyzed by KEGG pathway enrichment. D The protein-protein interaction network among proteins identified by mass spectrometry was constructed by PPI analysis in String Database. E WWP1(red) and NDFIP1 (green) colocalization in indicated cells was performed by confocal microscopy after immunofluorescence staining. F Interaction between WWP1 and NDFIP1 in HuCCT1, HCCC-9810, RBE cells were confirmed by co-IP assays followed by western blotting.

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