Fig. 7: WWP1 targeted NDFIP1 for ubiquitination and degradation. | Cell Death Discovery

Fig. 7: WWP1 targeted NDFIP1 for ubiquitination and degradation.

From: WWP1 upregulation predicts poor prognosis and promotes tumor progression by regulating ubiquitination of NDFIP1 in intrahepatic cholangiocarcinoma

Fig. 7

A The protein level of NDFIP1 in HuCCT1 cells with WWP1 knockdown was evaluated by western blotting. The protein levels of NDFIP1 in HCCC-9810 and RBE cells with WWP1 overexpression were measured by western blotting. B Using UbiBrowser Database (http://ubibrowser.ncpsb.org/), NDFIP1 was predicted to be ubiquitinated by WWP1. C HuCCT1 cells infected with the lentiviral shWWP1 and control lentivirus were incubated with 100 ug/ml cycloheximide (CHX) for the indicated time. The half-life of NDFIP1 was evaluated by western blotting. D The protein level of NDFIP1 in HuCCT1 cells with WWP1 knockdown, HCCC-9810, and RBE cells with WWP1 overexpression treated with 20 μM MG132, a potent proteasome inhibitor, for 4 h were analyzed by western blotting. E Ubiquitylation levels of NDFIP1 detected by Western blotting with anti-ubiquitin antibody after WWP1 knockdown and overexpression in HuCCT1, HCCC-9810, and RBE cell lines, respectively. F 293T cells were transfected with the indicated plasmids, the cell lysates were incubated with Flag-tagged beads and the ubiquitylation level of Flag-NDFIP1 was detected by western blotting with an anti-MYC antibody.

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